Titlebook: Meiosis; Volume 1, Molecular Scott Keeney Book 2009 Humana Press 2009 Arabidopsis thaliana.Budding and fission yeasts.Chromosome dynamics.

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Visual Markers for Detecting Gene Conversion Directly in the Gametes of ,s through the use of tetrad analysis. Here we describe a method for using tetrad analysis in the model plant . to detect and quantify gene conversion events – a resource unavailable in most other higher eukaryotic model systems.

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Genome-Wide Mapping of Meiotic DNA Double-Strand Breaks in ,emains covalently attached to DSB ends in certain mutant backgrounds. The second approach involves BND cellulose enrichment of the single-strand DNA (ssDNA) recombination intermediate formed by end-resection at DSB sites following Spo11 removal.

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Stabilization and Electrophoretic Analysis of Meiotic Recombination Intermediates in ,hen applied to the analysis of mutants for which genetic analysis of recombination is impossible, i.e. mutants that die during meiosis. This chapter describes three distinct methods to stabilize JMs against thermally driven dissolution as well as electrophoretic approaches to resolve and detect JMs at two well-characterized recombination hotspots.

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