Titlebook: Meiosis; Volume 1, Molecular Scott Keeney Book 2009 Humana Press 2009 Arabidopsis thaliana.Budding and fission yeasts.Chromosome dynamics.

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Stabilization and Electrophoretic Analysis of Meiotic Recombination Intermediates in , budding yeast has provided a wealth of information about the timing and mechanism of meiotic recombination. These assays are especially informative when applied to the analysis of mutants for which genetic analysis of recombination is impossible, i.e. mutants that die during meiosis. This chapter d

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Analysis of Chromatin Structure at Meiotic DSB Sites in Yeastsn but also ensures proper segregation of chromosomes. Meiotic recombination is initiated by DNA double-strand breaks that require many proteins including the catalytic core, Spo11. In this regard, like transcription and repair, etc., recombination is hindered by a compacted chromatin structure becau

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Analysis of Protein–DNA Interactions During Meiosis by Quantitative Chromatin Immunoprecipitation (qntroduction of DNA double-strand breaks (DSBs). The majority of DSBs, which mostly occur at so-called hotspots, have been located between cohesin binding sites. qChIP (chromatin immunoprecipitation quantified by real-time PCR) is a sensitive, accurate, and cost-efficient alternative to ChIP-on-Chip

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Analysis of Chromatin Structure at Meiotic DSB Sites in Yeastsdigestion of chromatin DNA and subsequent Southern blotting, is expected to provide information as to chromatin context around a hotspot. Moreover, by virtue of MNase preferentially targeting linker DNA, positions of several nucleosomes surrounding a hotspot can also be determined. Our protocol is a

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Book 2009unique chromosome dynamics of meiosis have fascinated scientists for well over a century, but in recent years there has been an explosion of new information about how meiotic chromosomes pair, recombine, and are segregated. Progress has been driven by advances in three main areas: (1) genetic identi

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