Titlebook: Electron Microscopy; Methods and Protocol John Kuo Book 2014Latest edition Springer Science+Business Media, New York 2014 (EM) data.Cryo-sp

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Single Particle Cryo-electron Microscopy and 3-D Reconstruction of Viruses,3-D reconstruction of icosahedral viruses has now reached near-atomic resolutions (3–4 ?). With comparable resolutions and more predictable outcomes, cryo-EM is now considered a preferred method over X-ray crystallography for determination of atomic structure of icosahedral viruses. At near-atomic r

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Ancient Eastern-Iranian Culturerom the tissues and resin. Microwave-assisted procedure can reduce specimen preparation time, but both conventional and microwave-assisted chemical fixation procedures produce artifacts. Cryo-specimen preparation involves with high-pressure freezing and freeze-substitution can minimize artifact form

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Gary W. Barrett,Terry L. Barrett,Jianguo Wurall improved visualization of ultrastructural details in biological specimens. Tannic acid and PPD improve membrane preservation, and malachite green is a phospholipid stain. All of these stains are compatible with aqueous fixatives and should be considered when the usual stains are not satisfactor