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Titlebook: Electron Microscopy; Methods and Protocol John Kuo Book 2014Latest edition Springer Science+Business Media, New York 2014 (EM) data.Cryo-sp

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發(fā)表于 2025-3-21 16:06:58 | 只看該作者 |倒序?yàn)g覽 |閱讀模式
書目名稱Electron Microscopy
副標(biāo)題Methods and Protocol
編輯John Kuo
視頻videohttp://file.papertrans.cn/307/306126/306126.mp4
概述Deals with biological and molecular research techniques based on TEM and SEM.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the exper
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Electron Microscopy; Methods and Protocol John Kuo Book 2014Latest edition Springer Science+Business Media, New York 2014 (EM) data.Cryo-sp
描述.This third edition of .Electron Microscopy: Methods and Protocols .expands upon the previous editions with current, detailed protocols on biological and molecular research techniques based on TEM and SEM as well as other closely related imaging and analytical methods. With new chapters on conventional and microwave assisted specimen, cryo-specimen preparation, negative staining and immunogold labelling techniques, DNA and RNA tracking using hybrization in TEM or Atomic Force Microscopy, TEM crystallography and cryo TEM 3D tomography, 3D tomography of resin embedded tissues using FIB-SEM, Correlative microscopy using fluorescence microscopy, confocal microscopy or immune labelling techniques for both TEM and FIB-SEM and Elemental and isotopic identification and their distribution in cells and tissues using TEM, SEM, Scanning Transmission Electron Microscopy (STEM), Secondary Ion Mass Spectrometry (SIMS) and Nano SIMS. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting
出版日期Book 2014Latest edition
關(guān)鍵詞(EM) data; Cryo-specimen; DNA and RNA tracking; Focussed Ion Beam (FIB); Scanning Electron Microscope (S
版次3
doihttps://doi.org/10.1007/978-1-62703-776-1
isbn_softcover978-1-4939-6073-6
isbn_ebook978-1-62703-776-1Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, New York 2014
The information of publication is updating

書目名稱Electron Microscopy影響因子(影響力)




書目名稱Electron Microscopy影響因子(影響力)學(xué)科排名




書目名稱Electron Microscopy網(wǎng)絡(luò)公開度




書目名稱Electron Microscopy網(wǎng)絡(luò)公開度學(xué)科排名




書目名稱Electron Microscopy被引頻次




書目名稱Electron Microscopy被引頻次學(xué)科排名




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書目名稱Electron Microscopy讀者反饋




書目名稱Electron Microscopy讀者反饋學(xué)科排名




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發(fā)表于 2025-3-22 02:37:24 | 只看該作者
A ‘Soldier of the Third International’paration of APS-mica surfaces and the preparation of samples for AFM imaging. The protocol for synthesis and purification of APS is also provided. The AFM applications are illustrated with examples of images of DNA and protein–DNA complexes.
地板
發(fā)表于 2025-3-22 07:41:04 | 只看該作者
History of Social Law in Germanyhods based on the enzymatic synthesis of the labeled nucleic acids chains that can be used for the detection of DNA or RNA molecules in situ are mentioned. In this respect, the technique enabling the enzymatic detection of the polyadenylated RNA sequences is described in detail.
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發(fā)表于 2025-3-22 16:53:24 | 只看該作者
Tracking DNA and RNA Sequences at High Resolution,hods based on the enzymatic synthesis of the labeled nucleic acids chains that can be used for the detection of DNA or RNA molecules in situ are mentioned. In this respect, the technique enabling the enzymatic detection of the polyadenylated RNA sequences is described in detail.
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發(fā)表于 2025-3-22 17:23:42 | 只看該作者
Biological Applications of Phase-Contrast Electron Microscopy,Zernike PC cryo-electron microscopy to biological systems such as protein molecules, virus particles, and cells, including single-particle analysis to delineate three-dimensional (3D) structures of protein and virus particles and cryo-electron tomography to reconstruct 3D images of complex protein systems and cells.
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發(fā)表于 2025-3-23 00:43:25 | 只看該作者
1064-3745 .Contains key notes and implementation advice from the exper.This third edition of .Electron Microscopy: Methods and Protocols .expands upon the previous editions with current, detailed protocols on biological and molecular research techniques based on TEM and SEM as well as other closely related im
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發(fā)表于 2025-3-23 02:52:48 | 只看該作者
https://doi.org/10.1007/978-3-031-36892-9mples in aluminum specimen carriers, cell cultures grown on Sapphire discs, suspensions for freeze-fracturing, and specimens for cryo-sectioning. Additionally, we include an advanced technique to freeze monolayer cell cultures on Sapphire discs with the Leica EM PACT2 HPF machine using a composite carrier.
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發(fā)表于 2025-3-23 06:07:14 | 只看該作者
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