Titlebook: Cytomegalovirus Protocols; John Sinclair Book 2000 Humana Press 2000 infectious diseases

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Construction of Recombinant Human Cytomegalovirus,e recombinant virus. Genetically modified viruses may also have applications in biotechnology and gene therapy. Such studies are well advanced for herpes simplex virus (HSV), a relative of human cytomegalovirus (HCMV), but have been relatively limited for HCMV itself.

Psa617

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Analysis of Cytomegalovirus Gene Expression by Transfection,nt patients (.–.). HCMV has a severely restricted host range in culture. It grows efficiently only in primary human diploid fibroblasts (HFF) and a few selected human cell lines (.–.). Monocytes serve as a major reservoir of latent HCMV in humans and HCMV can be grown in vitro in primary monocyte-derived macrophages (M/M) (.–.).