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Titlebook: Cytomegalovirus Protocols; John Sinclair Book 2000 Humana Press 2000 infectious diseases

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書目名稱Cytomegalovirus Protocols
編輯John Sinclair
視頻videohttp://file.papertrans.cn/243/242663/242663.mp4
叢書名稱Methods in Molecular Medicine
圖書封面Titlebook: Cytomegalovirus Protocols;  John Sinclair Book 2000 Humana Press 2000 infectious diseases
描述John Sinclair and a panel of expert investigators present a comprehensive collection of cellular and molecular techniques for the analysis of cytomegalovirus (CMV) biology and its pathogenetic mechanisms. The methods-all described in step-by-step detail with ready reproducibility in mind-range from basic virus culture to complex molecular analysis of CMV structure and function. Included are methods for CMV detection using both immunological and biological techniques, methods for analyzing fundamental aspects of the CMV infection cycle, and methods for analyzing T cell response to cytomegalovirus infection in the human host. Comprehensive and state-of-the-art, Cytomegalovirus Protocols provides investigators with a collection of the key methods that are illuminating not only the basic biology of this complex and intriguing human herpesvirus, but also its significant role in human infectious diseases and their emergent therapies.
出版日期Book 2000
關(guān)鍵詞infectious diseases
版次1
doihttps://doi.org/10.1385/1592592449
isbn_softcover978-1-61737-167-7
isbn_ebook978-1-59259-244-9Series ISSN 1543-1894 Series E-ISSN 1940-6037
issn_series 1543-1894
copyrightHumana Press 2000
The information of publication is updating

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Antiseizure Medication Interactionson (PCR) method first devised by Mullis and Faloona (.). PCR allows the in vitro amplification of HCMV DNA sequences by the simultaneous primer extension of complementary DNA strands. Similarly, reverse transcription-PCR (RT-PCR) allows the study of targeted gene expression, by reverse transcription
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https://doi.org/10.1007/978-3-030-82790-8tients, HCMV can cause severely debilitating colitis and retinitis; it is now an increasingly common cause of life-threatening pneumonitis in transplant patients (.–.). HCMV has a severely restricted host range in culture. It grows efficiently only in primary human diploid fibroblasts (HFF) and a fe
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Anthony McElligott,Jeffrey Herfoteins interact with those of the host cell. The most widely used method to identify interactions between viral and cellular proteins in the infected cell is that of co-immunoprecipitation; lysates from infected cells are treated with antibody which recognises, say, a viral protein of interest, and
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Anthony McElligott,Jeffrey Herf capacity for more than 200 different proteins (.,.). The genes encoding those proteins are expressed coordinately during the replication of this virus (.). Although posttranscriptional regulation has been described for HCMV, transcriptional regulation by promoter activation is believed to play a ke
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發(fā)表于 2025-3-23 08:46:59 | 只看該作者
Antisemitism Before and Since the Holocaustve inhibitors of HCMV replication. Selectivity means that the window between the concentrations that block viral replication on the one hand and those that affect host cell functions on the other is sufficiently wide. Because HCMV induces an obvious cytopathic effect (CPE) in human fibroblast cultur
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