Titlebook: Cancer Cell Signaling; Methods and Protocol David M. Terrian Book 2003 Humana Press 2003

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Generation of Axons and Dendrites of, protein-protein interactions in cells that do not typically occur, or are weaker, under normal redox conditions. In this chapter, I describe a method to measure the strength of protein-protein interactions that may be enhanced during oxidative stress using the yeast two-hybrid system.

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Regressive Phenomena: Refining Connectionssion. One is based on BrdU incorporation (i.e., DNA replication) and the other on flow cytometry. Together, this triad of techniques is sufficient to determine the action of tumor suppressors and other antiproliferative agents.

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Paradox

Neuroscience in the 21st Centuryce in human neuroblastoma cells to doxorubicin, a topoisomerase-2 inhibitor, and to MDL-28842, an inhibitor of S-adenosylhomocysteine hydrolase, and reductions in the activation and nuclear translocation of MAPk.

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Eric D. Finegood,Gregory E. Millere-modified antisense ODNs were transferred into prostate cancer cells with high efficiency, effectively inhibiting the expression of endogenous protein kinase C-epsilon and the androgen-independent (AI) proliferation of several independent human prostate cancer cell lines.

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Introduction to the Study of Stress,ocus on basic tissue culture techniques which are used to elucidate signal transduction pathways. Furthermore, this chapter will provide a general background for understanding how gene transfer techniques can be used to elucidate signal transduction pathways as well as various pitfalls commonly encountered with their usage.