Titlebook: Volume Microscopy; Multiscale Imaging w Irene Wacker,Eric Hummel,Rasmus Schr?der Book 2020 Springer Science+Business Media, LLC, part of Sp

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Large-Scale Automated Serial Section Imaging with a Multibeam Scanning Electron Microscope,on microscope. For the ZEISS MultiSEM, the special constraints of operating a multibeam scanning electron microscope on larger samples than traditionally possible require adapted sample preparation techniques for successful imaging. In this chapter, we introduce multibeam scanning electron microscop

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Using X-Ray Microscopy to Increase Targeting Accuracy in Serial Block-Face Scanning Electron Microslume EM). By providing a means of nondestructively imaging EM specimens prior to performing volume EM, XRM allows the investigator to pinpoint specific regions of interest (ROIs) for imaging. In addition, given the excellent contrast and resolution that can be achieved with XRM when specimens are st

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FIBSEM Analysis of Interfaces Between Hard Technical Devices and Soft Neuronal Tissue,uronal answers to these stimuli vice versa. 3D reconstruction of such biological technical interfaces is helpful to gain morphological insights to these interfaces. Questions like whether and how cellular structures do interact with the technical surface are important to interpret the functionality

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Transforming FIB-SEM Systems for Large-Volume Connectomics and Cell Biology,l focused ion beam scanning electron microscopy (FIB-SEM) offers unique benefits such as high resolution (<10?nm in ., ., and .), robust image alignment, and minimal artifacts for superior tracing of neurites. However, its prevailing deficiencies in imaging speed and duration cap the maximum possibl

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Image Processing for Volume Electron Microscopy,l analysis almost infeasible, and data storing and processing challenging. Specialized infrastructure and software was therefore developed during the last decade to address these problems, ranging from distributed and versioned 3D image stores to deep neural network architectures optimized for the s

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Forget About Electron Micrographs: A Novel Guide for Using 3D Models for Quantitative Analysis of Dmon. Optical, or physical sectioning, and subsequent imaging of biological tissue at high resolution, offers the chance to postprocess, segment, and reconstruct micro- and nanoscopical structures, and then reveal spatial arrangements previously inaccessible or hardly imaginable with simple, single s

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