Titlebook: Vertebrate Embryogenesis; Embryological, Cellu Francisco J. Pelegri Book 2019Latest edition Springer Science+Business Media, LLC, part of S

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1064-3745 advice from the expertsThis detailed volume explores animal embryogenesis in a way that aims to foster cross-model work and ideas by presenting methods that can be applied across laboratories and species boundaries. Improved protocols with updated advances in key traditional model systems are includ

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Immunofluorescence of Microtubule Assemblies in Amphibian Oocytes and Early Embryos,ers, chromosomes, and nuclei in whole-mount embryos, even when they are hundreds of micrometers removed from the embryo’s surface. Though the described methods were optimized for microtubule assemblies, they have also proven useful for the visualization of other proteins.

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Manipulation of Developmental Function in Turtles with Notes on Alligators,en adapted and optimized from methods used for frog, chick, and mouse embryos, it is likely that they could be used for other reptilian species. Notes are included for alligator embryos where appropriate.

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Isolation of Zebrafish Balbiani Bodies for Proteomic Analysis,ugh a syringe, and then used a fine injection needle to wick up Balbiani bodies by capillary action with minimal buffer solution. Using this protocol we collected sufficient material for proteomic analysis of the zebrafish Balbiani body.

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Experimental Manipulation of Ploidy in Zebrafish Embryos and Its Application in Genetic Screens,haploid or tetraploid states. We also describe methodologies to verify the ploidy of embryos and the utility of ploidy manipulation in expediting the isolation of mutations using both forward and reverse genetic strategies in zebrafish.

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Thao Nguyen,Timothy J. Mitchison,Martin Wührhsvollen Randbedingungen auf Baustellen und die im Bauwesen typische Unikatbauweise unterliegen Beton-3D-Druckverfahren besonderen Herausforderungen. Die innovativen Technologien stehen darüber hinaus im wirtschaftlichen Wettbewerb zu konventionellen Bauverfahren. Um die Wirtschaftlichkeit und Proze

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