Titlebook: Vascular Hyperpermeability; Methods and Protocol Binu Tharakan Book 2024 Springer Science+Business Media, LLC, part of Springer Nature 2024

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Measurement of Blood-Brain Barrier Hyperpermeability Using Evans Blue Extravasation Assay,most popular methods for studying BBB integrity and permeability in rodent models of brain disorders. Under normal physiological conditions, the BBB is impermeable to albumin, so Evans Blue when injected intravenously binds to serum albumin and remains restricted within blood vessels. In traumatic a

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Measurement of Transendothelial Electrical Resistance in Blood-Brain Barrier Endothelial Cells, homeostasis. Brain microvascular endothelial cells are primary components of the blood-brain barrier. Transendothelial electrical resistance (TEER) is the electrical resistance across a cellular monolayer such as the brain microvascular endothelial cell monolayers. Measurement of TEER is considered

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Book 2024aboratory setting. The chapters in this book cover topics such as determination of solute permeability of microvascular endothelial cell monolayers in vitro; evaluation of barrier integrity used in a two-layered microfluidic device mimicking the blood-brain barrier; isolation and culture of human um

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978-1-0716-3431-8Springer Science+Business Media, LLC, part of Springer Nature 2024

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