Titlebook: VEGF Signaling; Methods and Protocol Lorna Fiedler Book 2015 Springer Science+Business Media New York 2015 Cardiovascular Development.Lymph

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Identification of Receptor Tyrosine Kinase Inhibitors Using Cell Surface Biotinylation and Affinity relevant readout of inhibitor activity. In this example, we use the VEGF-A–VEGFR-2 axis and the well-characterized tyrosine kinase inhibitor sunitinib as a working model; however this technique is highly applicable for the identification of inhibitors to other receptor tyrosine kinases.

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1064-3745 ation advice from the experts.This volume provides a collection of protocols for studying and manipulating VEGF signaling pathways in vitro and in vivo, and in particular, aims to present a range of both firmly established and newly emerging technologies, including those that are amenable to aiding

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Chromatin Immunoprecipitation Assay: Examining the Interaction of NFkB with the VEGF Promotern cofactors, DNA replication factors, and DNA repair proteins. Here we describe a useful ChIP-qPCR protocol to examine the interaction of NFkB with the VEGF promoter in adult rat primary cardiomyocytes that have been mechanically stretched after attaching to the extracellular matrix protein laminin.

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