Titlebook: Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC); Methods and Protocol Bettina Warscheid Book 2014 Springer Science+Business

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Book 2014 Acids in Cell Culture (SILAC): .Methods and Protocols .will serve students and experienced scientists alike as a valuable reference of how to make use of the SILAC technology for their own research..

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Fifteen Years of Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC),l labeling as well as to clinical applications—in the form or spike-in or super-SILAC. New formats and applications for SILAC labeling continue to be developed, for instance for protein-turnover studies.

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Applying SILAC for the Differential Analysis of Protein Complexes, binders. Besides pruning out contaminants, high-resolution MS data combined with quantitative proteomics software allow the comparative analysis of protein interaction patterns of different protein variants, for example mutated versus normal protein variant or of regulatory changes in a given protein complex due to different states of activity.

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