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Titlebook: Seed Dormancy; Methods and Protocol Naoto Kawakami,Kazuhiro Sato Book 2024 The Editor(s) (if applicable) and The Author(s), under exclusive

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發(fā)表于 2025-3-30 09:52:26 | 只看該作者
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發(fā)表于 2025-3-30 12:54:55 | 只看該作者
Genome Editing in Commercial Wheat Varieties: Use of , to Lengthen Seed Dormancyarticle bombardment (iPB) technique for gene editing in wheat that utilizes shoot apical meristems (SAMs) as a target tissue. Since the method does not require in vitro culture, it can therefore be used on recalcitrant varieties. In this chapter, we describe in detail the steps used in the iPB metho
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發(fā)表于 2025-3-30 19:56:19 | 只看該作者
Genetic, Epigenetic, and Environmental Control of Seed Dormancy and Germinationon in model plants and economically important crop species. This chapter focuses on temperature as an environmental factor and discusses the genetic and epigenetic mechanisms of dormancy and germination.
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發(fā)表于 2025-3-30 20:59:44 | 只看該作者
Assessment of Dormancy Level and Germination Ability of Seeds with Physiological Dormancytest mainly for physiological and molecular genetic studies with Arabidopsis. This protocol can be applied for other plant species with relatively small seeds and for various studies to analyze the effect of light, phytohormones, and other chemicals in seed germination.
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發(fā)表于 2025-3-31 16:41:46 | 只看該作者
Confocal Imaging of Seedshods. Here, we describe two techniques to imaging the three-dimensional structure of . seeds by confocal laser scanning microscopy. Both procedures, while differing in their time of execution and resolution, are based on cell wall staining of seed tissues with fluorescent dyes.
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