Titlebook: Ribozymes and siRNA protocols; Mouldy Sioud Book 2004Latest edition Humana Press 2004

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Identification of Efficient Cleavage Sites in Long-Target RNAs,. After two rounds, the active preselected library pool is incubated at 37°C with target RNA in the presence of magnesium, and cleavage products are directly identified on sequencing gels. The protocol identifies highly active hRz, which typically have ..s of 20–80 n., and ../.. values of 10..

Forehead-Lift

Ribozyme Expression Systems,antly higher than that of ribozymes that remained in the nucleus. This chapter examines the methods for construction of the appropriate vector that can produce tRNA-driven ribozymes with high-level intracellular activity and for analysis of the constructs in cells.

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Real-Time Monitoring of RNA and DNA Reactions by Fluorescence Detection,ase or a second dye). Applications include detailed kinetic studies and optimizing reactions with a wide range of conditions and highly specific nucleic acid sequence detections, challenging other hybridization-based methods. Special applications are the introduction of catalytic nucleic acids for r

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