Titlebook: Ribozyme Protocols; Philip C. Turner Book 1997 Springer Science+Business Media New York 1997

半圓鑿

Arnold Hampel,Mary Beth De Young,Scott Galasinski,Andrew Siwkowski denen des kristallinen Siliziums abweichen, berücksichtigt werden. Nahezu alle diese abweichenden Eigenschaften lassen sich von einem grundlegenden Unterschied zwischen Si und GaAs ableiten: GaAs ist ein ., Si dagegen . (s. Abb. 3.2). Daraufhin verl?uft der Absorptionskoeffizient α(λ) des GaAs über

狂熱文化

Ribozymes,ic RNA molecules, or ribozymes, to carry out their specific reactions has led to the realization that ribozymes can easily be manipulated to act on novel substrates. These custom-designed RNAs have great potential as therapeutic agents and are becommg a powerful tool for molecular biologists.

語言學

Chauvinistic

寡頭政治

PCR-Based Construction of Long Hammerhead Ribozymes,isense RNA containing the hammerhead catalytic core that will cleave the target of interest at a GUC sequence. All discussion will focus on the hammerhead motif, although, in principle, the hairpin motif could be incorporated instead.

Digest

grieve

Ribozymes, primarily passive carriers of genetic information, can carry out some functions previously ascribed to proteins. Some RNAs are capable of acting as enzymes even in the complete absence of proteins. The reactions observed include cleaving themselves or other RNA molecules, ligation, and .-splicing.

Arb853

Computer-Aided Calculation of the Local Folding Potential of Target RNA and Its Use for Ribozyme Deleavable motif. In the case of hammerhead ribozymes, this is a specific base triplet. Even though it has been described that there are 12 conceivable motifs recognized by hammerhead ribozymes that are cleaved with different efficiencies (.,.), a typical long-chain target RNA provides a large number

Monotonous

Computational Approaches to the Identification of Ribozyme Target Sites,rofound. Although we are only beginning to understand the underlying reasons for this extreme variation, we can identify a number of variables that might well be significant in the process, and some of these can be modeled computationally. It must be emphasized, however, that only well-designed and

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