Titlebook: Riboregulator Design and Analysis; James Chappell,Melissa K. Takahashi Book 2022 The Editor(s) (if applicable) and The Author(s), under ex

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Efficient Method to Identify Synthetic Riboswitches Using RNA-Based Capture-SELEX Combined with In otide through structural changes. These sequences get reverse transcribed, PCR amplified, and used for the following selection rounds. In this publication, we present a detailed protocol for Capture-SELEX, followed by screening in yeast to identify aptamers suitable for the design of synthetic riboswitches.

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RNA Design Principles for Riboswitches that Regulate RNase P-Mediated tRNA Processing,e biochemical analysis for the verification of the implemented mechanism. Furthermore, we discuss the applicability of the presented biochemical in vivo and in vitro methods for the characterization of other artificial riboswitches.

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Single-Molecule FRET Studies of RNA Structural Rearrangements and RNA-RNA Interactions,f suitable RNA substrates for sRNA-mRNA annealing reactions, preparation of internally labeled mRNA for detecting conformational changes in the target, and key steps of the data analysis. These protocols can be adapted to other RNA-binding proteins that chaperone RNA interactions.

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RNP-Based Control Systems for Genetic Circuits in Synthetic Biology Beyond CRISPR,de users a streamlined method for identifying an RNP control system most useful to their own work. Lastly, we discuss future applications of RNP control systems and how they can be utilized to address the current grand challenges of the synthetic biology community.

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