Titlebook: Retinoid Protocols; Christopher P. F. Redfern Book 1998 Springer Science+Business Media New York 1998

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Detection and Measurement of Retinoic Acid Production by Isolated Tissues Using Retinoic Acid-Sensit in early embryonic development has been suggested from studies in which exogenous retinoids were administered to developing vertebrate embryos .. These treatments resulted in morphological alterations in a number of diverse tissues including craniofacial structures, limbs, the hindbrain of the cent

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Immunohistochemistry for CRBPs and CRABPscid-binding proteins (CRABP I and II), have been known for a long time (.). Yet, the precise roles of these proteins in retinoid physiology remains poorly defined. One of the major reasons for this has been the difficulties of generating high-quality antibodies to facilitate their detection in tissu

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Whole-Mount , Hybridization of Mouse Embryos Exposed to Teratogenic Levels of Retinoic Acidd, and then incubated with a labeled riboprobe. Once excess probe has been removed, the hybridized riboprobe that remains is detected by means appropriate to the label. Variations of the technique have been extensively used to analyze the expression patterns of many genes in the tissues of a wide va

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Methods for Producing Recombinant Human Cellular Retinaldehyde-Binding Proteinction of 11-.-retinol with visual-cycle enzymes in the retinal pigment epithelium (RPE) (.). This 36-kDa protein is remarkably stereo selective in recognizing the isomer of retinaldehyde mvolved in vision, and carries 11-.-retinaldehyde or 11-.-retinol as physiological ligands (.). Notably, retinoid

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Expression and Purification of CRABPs from ,ins (CRABPs) also affect the ability of retinoids to initiate biological signals (., .). holo-CRABP I sequesters retinoic acid (RA) with a .. value that may be <1 n., and serves as a high-affinity (.. ~2 n.), efficient substrate of RA metabolism, thereby controlling not only the steady-state concent

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Purification and Fluorescent Titration of Cellular Retinol-Binding Proteinotein II (CRBP II) (.., and references therein). CRBP is present in a wide variety of tissues, including liver, kidney, and testis. The basic feature of CRBP is the retinol-protein recognition, through which a drastically higher stability for CRBP-bound retinol relative to uncomplexed retinol is ach