Titlebook: Retinal Development; Methods and Protocol Shu-Zhen Wang Book 2012 Springer Science+Business Media, LLC 2012 DNA methylation.ERG (function)

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In Vitro Biochemical Assays to Monitor Rhodopsin Functionght activation catalyzes the activation of its G-protein transducin. The first step in resetting rhodopsin and the phototransduction cascade involves the phosphorylation of light-active rhodopsin by rhodopsin kinase. Here, we describe assays to monitor the function of rhodopsin or rhodopsin mutants.

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Studying the Generation of Regenerated Retinal Neuron from Müller Glia in the Mouse Eyerce of neuronal regeneration by de novo neurogenesis. Although spontaneous regeneration in mammals is absent after retinal damage, we discovered that certain manipulations stimulate a limited regenerative program in adult mice. This allows the study of cellular and molecular barriers that limit rege

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Subretinal Delivery and Electroporation in Pigmented and Nonpigmented Adult Mouse Eyese eye with naked plasmids. However, both techniques, subretinal injection and electroporation, present some technical challenges that require skill on the part of the surgeon to prevent untoward damage to the eye. Here we describe methods that we have used for the past 10 years (Johnson et al. Mol V

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