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Titlebook: Regulatory Non-Coding RNAs; Methods and Protocol Gordon G. Carmichael Book 2015 Springer Science+Business Media New York 2015 Fluorescence

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發(fā)表于 2025-3-21 17:23:04 | 只看該作者 |倒序瀏覽 |閱讀模式
書目名稱Regulatory Non-Coding RNAs
副標題Methods and Protocol
編輯Gordon G. Carmichael
視頻videohttp://file.papertrans.cn/826/825748/825748.mp4
概述Includes cutting-edge methods and protocols on non-coding RNAs.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes su
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Regulatory Non-Coding RNAs; Methods and Protocol Gordon G. Carmichael Book 2015 Springer Science+Business Media New York 2015 Fluorescence
描述.Regulatory Non-Coding RNAs: Methods and Protocols. offers a collection of methods for those interested in the discovery, localization, and functional analysis of these non-coding transcripts that have the potential and ability to orchestrate and control gene expression. After a review of the field, this detailed volume continues with methods useful for the study of siRNAs, microRNAs and their targets, techniques concerned with long non-coding RNAs, as well as studies of the critical parameters of functional non-coding RNA protein-RNA interactions and the environment in which they act. Written for the highly successful .Methods in Molecular Biology. series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips for troubleshooting and avoiding known pitfalls..Dependable and easy to use, .Regulatory Non-Coding RNAs: Methods and Protocols. provides a current, state-of-the-art collection of methods and approaches that will be of value to researchers in this expanding and fascinating field..
出版日期Book 2015
關(guān)鍵詞Fluorescence in situ hybridization; Long non-coding RNAs; MicroRNA targets; Non-coding transcriptome; Pr
版次1
doihttps://doi.org/10.1007/978-1-4939-1369-5
isbn_softcover978-1-4939-4184-1
isbn_ebook978-1-4939-1369-5Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2015
The information of publication is updating

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發(fā)表于 2025-3-21 22:41:00 | 只看該作者
https://doi.org/10.1007/978-1-4939-1369-5Fluorescence in situ hybridization; Long non-coding RNAs; MicroRNA targets; Non-coding transcriptome; Pr
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A Biochemical Approach to Identify Direct MicroRNA Targets,y the tumor suppressor miRNA miR-34a. This method involves transfection of cells with biotinylated miRNA mimics, streptavidin pulldown, RNA isolation, and qRT-PCR. The protocol in this chapter describes these steps and the issues that should be considered while designing such pulldown experiments.
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RNA Fluorescence In Situ Hybridization in Cultured Mammalian Cells, study of the distribution and function of lncRNAs is RNA fluorescence in situ hybridization (RNA-FISH). The protocols presented in this chapter describe this method in detail and also mention a number of critical points that must be considered when performing this technique.
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發(fā)表于 2025-3-22 18:14:51 | 只看該作者
Rapid and Efficient Elimination of Specific Nuclear Noncoding RNAs in Mammalian Cells with Antisensons have yet to be elucidated. Here, we describe a method for the rapid and efficient elimination of specific nuclear noncoding RNAs in mammalian cells using chemically modified antisense oligonucleotides, which may facilitate the functional assessment of nuclear ncRNAs.
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Regulatory Non-Coding RNAs978-1-4939-1369-5Series ISSN 1064-3745 Series E-ISSN 1940-6029
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