Titlebook: Recombinant Glycoprotein Production; Methods and Protocol Virgínia Pican?o-Castro,Kamilla Swiech Book 2018 Springer Science+Business Media,

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Bioreactor-Based Production of Glycoproteins in Plant Cell Suspension Culturests in anticancer drugs. A specific glycan profile is often necessary to achieve certain desirable activities, such as the effector functions of an antibody, receptor binding or a sufficient serum half-life. However, many expression systems produce glycan profiles that differ substantially from the p

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Fed-Batch CHO Cell Culture for Lab-Scale Antibody Productionary (CHO) cells. Developing and optimizing this process in the lab is crucial for establishing process knowledge, which enables rapid and predictable tech-transfer to manufacturing scale. In this chapter, we describe stepwise how to carry out fed-batch CHO cell culture for lab-scale antibody product

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Strategies to Develop Therapeutic N- and O-Hyperglycosylated Proteinsroteins with the aim of producing biotherapeutics with improved pharmacodynamic and pharmacokinetic properties. In this chapter, a detailed description of the steps routinely performed to generate new proteins having high content of N- and/or O-glycosyl moieties is carried out. The rational strategy

ANTIC

Expression of Glycosylated Proteins in Bacterial System and Purification by Affinity Chromatographyvaccines against pathogens. In this case, a protein carrier conjugated with glycosides is used to produce intense stimulation of the immune system. Glycoconjugate vaccines account for 35% of the global vaccine market, and consequently, several biotechnological companies have developed products for t

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Purification Methods for Recombinant Factor VIII Expressed in Human Liver SK-Hep Cellsry short half-live and need a fast and efficient purification chain. Here, we describe a simple purification sequence using multimodal Capto MMC, affinity FVIII select and ion exchange SP-Fastflow chromatography steps without subjecting the target molecule to mechanical and temperature stress, separ

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