Titlebook: Receptor Signal Transduction Protocols; Gary B. Willars,R. A. John Challiss Book 20042nd edition Humana Press 2004

無(wú)意

厚顏無(wú)恥

Identification of G-Protein-Coupled Receptor mRNA Expression by Northern Blotting and , Hybridizatis the ability to identify genetic splice variants, or to study multiple RNA molecules sequentially in the same tissue samples..These protocols give step-by-step instructions for the performance of these techniques, and the analysis of the data that can be obtained using them.

合群

Generation of Model Cell Lines Expressing Recombinant G-Protein-Coupled Receptors,ran, cationic lipids, and electroporation), allowing their transient or stable expression in diverse cell lines. This chapter gives a brief overview of the different techniques and provides methodology for the generation of transiently transfected cells and for selection, isolation and maintenance of stable transfected cell lines.

妨礙

excrete

皮薩

Radioligand-Binding Methods for Membrane Preparations and Intact Cells,ing assays for G-protein-coupled receptors in membranes and intact cells are presented for these types of experiments. Detailed procedures for analysis of the data obtained from these experiments are also given.

Commodious

高爾夫

擔(dān)憂(yōu)

delta-waves

Radioligand-Binding Methods for Membrane Preparations and Intact Cells,igand-binding experiments: (1) saturation experiments from which the affinity of the radioligand for the receptor and the binding site density can be determined; (2) inhibition experiments from which the affinity of a competing, unlabeled compound for the receptor can be determined; and (3) kinetic