Titlebook: Rational Drug Design; Methods and Protocol Yi Zheng Book 2012 Springer Science+Business Media New York 2012 ATP binding.GTPases.Structure b

參考書目

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Rational Drug Design978-1-62703-008-3Series ISSN 1064-3745 Series E-ISSN 1940-6029

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懦夫

https://doi.org/10.1007/978-1-62703-008-3ATP binding; GTPases; Structure based virtual screening; anti-sense microRNA; drug discovery; drug resist

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Virtual Ligand Screening Combined with NMR to Identify Dvl PDZ Domain Inhibitors Targeting the Wnt Virtual ligand screening is a powerful technique to identify potential hits of targets and to increase hit rates. Here, we describe how we used this technique combined with NMR .N HSQC experiments to obtain small molecules that bind to the PDZ domain of Dvl targeting the Wnt signaling pathway.

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Determination of the Kinetics and Thermodynamics of Ligand Binding to a Specific Inactive ConformatRecent interest in inactive kinase conformations has generated the need to develop new biochemical tools to study them. Here, we describe the use of a fluorescent probe that selectively and potently binds to a specific inactive conformation of protein kinases. This allows for the thermodynamics and kinetics of ligand binding to be determined.

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Yi ZhengAids scienctist in further study of rational drug design.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes suppleme

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