Titlebook: Radiation Cytogenetics; Methods and Protocol Takamitsu A. Kato,Paul F. Wilson Book 2019 Springer Science+Business Media, LLC, part of Sprin

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DNA Damage Focus Formation Assay,and breaks are most toxic to cells. DSBs can form mutations, chromosome aberrations, and cell killing. Although DSBs in cells can be detected directly by neutral elution, pulse field gel electrophoresis, and premature chromosome condensation, recent technologies like cellular immunocytochemistry-bas

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PNA Telomere and Centromere FISH Staining for Accurate Analysis of Radiation-Induced Chromosomal Ab-based cytogenetic analysis is labor-intense and time-consuming. Moreover, the disadvantage of Giemsa based chromosome analysis is a potential poor reproducibility when researchers are not fully trained for analysis. These problems come from analysis of morphological abnormality of chromosomal aberr

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Modified PNA Telomere and Centromere FISH Protocols,ely used to identify chromosome aberrations and have been shown to greatly aid in biodosimetery assays involved in identifying dicentrics. Traditional techniques have required the heat denaturing of the DNA in formamide followed by multiple hours at moderated temperatures to allow the probe to hybri

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Reciprocal Translocation Analysis with Whole Chromosome Painting for FISH,ining and require special staining methods. Moreover, an easy method to identify nondetectable chromosomal aberrations, such as symmetrical inter-chromosomal translocations, is available: whole chromosome fluorescence in situ hybridization (FISH) staining. Asymmetrical translocations such as dicentr

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Animal Lymphocyte Metaphase Chromosome Preparation, general procedure evaluating metaphase chromosomes is similar to the human blood technique except for a minor modification in the initial culture. This chapter will introduce basic mouse, rat, rabbit, dog, cat, cow, horse, goat, pig, and wild boar venipuncture and blood sampling techniques for metaphase chromosome preparation and analysis.

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