Titlebook: RNA-Protein Complexes and Interactions; Methods and Protocol Ren-Jang Lin Book 2023Latest edition The Editor(s) (if applicable) and The Aut

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1064-3745 oritative and cutting-edge,. RNA-Protein Complexes and Interactions: Methods and Protocols, Second Edition .aims to be comprehensive guide for researchers in the field..978-1-0716-3193-5978-1-0716-3191-1Series ISSN 1064-3745 Series E-ISSN 1940-6029

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978-1-0716-3193-5The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines

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https://doi.org/10.1007/978-1-0716-3191-1snRNPs; Crispr-Cas9; immunoprecipitation; Mass-spec; Ribo-proteomics

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Ren-Jang LinIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts

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A Simple Method for the Detection of Wybutosine-Modified tRNAPheGAA as a Readout of Retrograde tRNAof the movement of tRNAs and the proteins mediating these movements can be difficult. Here, we describe an easy and cost-effective assay to discover genes that are involved in two specific tRNA trafficking events, retrograde nuclear import and nuclear re-export for yeast, .. This assay, referred to