Titlebook: RNA-Protein Complexes and Interactions; Methods and Protocol Ren-Jang Lin Book 2016 Springer Science+Business Media New York 2016 RNA-bindi

COUCH

Overdose

jovial

Loading of Argonaute Protein with Small Duplex RNA in Cellular Extracts,to regulate gene expression through RNAi they must be loaded, or “programmed,” with a single strand of small RNA. Natural small RNAs are typically double-stranded duplexes that require additional factors for efficient and specific loading into Ago proteins. Here, a protocol is described for investig

Ptsd429

Phenothiazines

fastness

Purification and Functional Reconstitution of Box H/ACA Ribonucleoprotein Particles,. In eukaryotes and archaea, RNA pseudouridylation is catalyzed largely by box H/ACA ribonucleoproteins (RNPs), a distinct group of RNA–protein complexes each consisting of a unique RNA and four common proteins. The RNA component of the complex serves as a guide that base-pairs with its substrate RN

CAMEO

,Northwestern Blot Analysis: Detecting RNA–Protein Interaction After Gel Separation of Protein Mixtu to prepare .P-labeled RNA probes and to use them to assay for RNA–protein interactions after partially purified protein preparations are resolved on denaturing SDS-polyacrylamide gels. The method can unambiguously determine whether the protein of interest can directly and independently bind RNA eve

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內(nèi)部

Identification of Endogenous mRNA-Binding Proteins in Yeast Using Crosslinking and PolyA Enrichmentation of the complete set of mRNA-binding proteins is a key step in understanding the regulation of gene expression. Herein, we describe a method for identifying yeast mRNA-binding proteins in a systematic manner using UV crosslinking, purification of polyA(+) mRNAs under denaturing conditions, and