Titlebook: RNA Vaccines; Methods and Protocol Thomas Kramps,Knut Elbers Book 2017 Springer Science+Business Media New York 2017 recombinant viruses.se

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Electroporation of mRNA as Universal Technology Platform to Transfect a Variety of Primary Cells wittion protocol and the experience we have gathered with this method. The advantages of the transfection method presented here are: (1) easy adaptation to different cell types, (2) scalability from 10. to approximately 10. cells per shot, (3) high transfection efficiency (80–99 %), (4) homogenous prot

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Enhanced Delivery of DNA or RNA Vaccines by Electroporationty, pulse length, pulse width and drug product formulation) majorly impact the efficiency of nucleic acid delivery. We provide an overview of DNA/RNA vaccination in the muscle of mice. Our results suggest that the technique is safe and effective and is highly applicable to a research setting as well

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The European Regulatory Environment of RNA-Based Vaccineson whether RNA-based vaccines are directed against tumors or infectious disease, they are formally considered gene therapy products or not, respectively. Besides an overview on the current clinical use of mRNA vaccines in various therapeutic areas a detailed discussion of the current regulatory situ

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ions in which electrostatic accelerators are the preferred tool for accelerator-based investigations. Since some topics are common to all types of accelerators, .Electrostatic Accelerators. will als978-3-642-06309-1978-3-540-27095-9Series ISSN 1611-1052 Series E-ISSN 2365-0877

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ions in which electrostatic accelerators are the preferred tool for accelerator-based investigations. Since some topics are common to all types of accelerators, .Electrostatic Accelerators. will als978-3-642-06309-1978-3-540-27095-9Series ISSN 1611-1052 Series E-ISSN 2365-0877

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1064-3745 ation advice from the experts.Includes supplementary materia.This volume provides an overview of the field and practical hints for vaccinologists in academia and industry. Chapters provide protocols on self-replicating RNA vectors, non-replication mRNA vectors, adjuvantation and delivery, and precli