Titlebook: RNA Structure and Dynamics; Jienyu Ding,Jason R. Stagno,Yun-Xing Wang Book 2023 This is a U.S. government work and not under copyright pro

dendrites

SEMI

Bother

Exploring the Binding Process of Cognate Ligand to , Adenine Riboswitch Aptamer by Using Explicit Sg. Therefore, the biological functions of riboswitches mainly depend on the switching of secondary and three-dimensional structures in the presence and absence of the metabolite ligands. However, the binding mechanisms of cognate ligands to riboswitches are still not well understood. Here, we have i

Pigeon

,Prediction of Dynamic RBP–RNA Interactions Using PrismNet,nd the functions of the RBP in posttranscriptional regulation. However, the prediction of RBP binding sites in vivo remains challenging. Tools that predict RBP–RNA interactions using sequence and/or predicted structures cannot reflect the exact state of RNA in vivo. PrismNet, which uses both sequenc

軍火

Ingenuity

Ophthalmologist

,Combining Biophysical Methods for Structure–Function Analyses of RNA in Solution,hanges that affect transcription or translation. Although several biophysical methods have been employed to study such RNAs, the utility of any one single method is limited. Hybrid approaches, therefore, are essential to better characterize these intrinsically dynamic molecules and elucidate their r

絕種

Near-Atomic Resolution Cryo-EM Image Reconstruction of RNA,ation. Protein, protein–lipid, and protein–nucleic acid complexes can now be routinely resolved by cryo-EM single-particle analysis (SPA) to near-atomic or atomic resolution. Here we describe the structure determination of pure RNAs by SPA, from cryo-specimen preparation to data collection and 3D re

步兵

Auto-DRRAFTER: Automated RNA Modeling Based on Cryo-EM Density,y (cryo-EM) allow structure determination of RNAs and ribonucleoprotein (RNP) complexes. However, limited global and local resolutions of RNA cryo-EM maps?pose great challenges in tracing RNA coordinates. The Rosetta-based “auto-DRRAFTER” method builds RNA models into moderate-resolution RNA cryo-EM

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