Titlebook: RNA Silencing; Methods and Protocol Gordon G. Carmichael Book 2005 Humana Press 2005 DNA.Vivo.gene expression.genes.molecular biology.regul

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M. Florian Mette,Werner Aufsatz,Tatsuo Kanno,Lucia Daxinger,Philipp Rovina,Marjori Matzke,Antonius J-selective, and line-narrowed spectra of chromophores doped into matrices; the interplay between states of ligand-centered .ππ* and .MLCT character; localization versus delocalization behavior; radiative decay properties; zero-field splittings; spin-lattice relaxations via direct and Orbach mechanis

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Peng Liu,Mary Louise Stover,Alexander Lichtler,David W. Roweengineering tools for improving pattern control and array efficiency including lattice selection, subarrray technology and pattern synthesis. Equations and figurers quantify the phenomena being described and provide the reader with the tools to tradeoff various performance features. The discussions

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Identification of Components of RNAi Pathways Using the Tandem Affinity Purification Method,nctions by an endogenous pathway important for normal development in many organisms, including gene regulation, virus resistance, and chromatin remodeling (.–.). Although some components of the RNAi cellular machinery have been identified, the overall picture is far from clear. We describe the tande

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Down-Regulating Gene Expression by RNA Interference in ,, epitope tagging can also be effected through homologous recombination (.). For general reviews of the peculiarities of trypanosome gene expression and RNA processing, see .. and ., and for pre-RNAi methods, such as inducible gene expression and knockouts by homologous recombination, see ... A few r

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Inhibition of Gene Expression In Vivo Using Multiplex siRNA,ate the various phases of angiogenesis. A major regulator of angiogenesis, vascular endothelial growth factor (VEGF), was upregulated in tumor angiogenesis, and inhibition of this factor with a specific monoclonal antibody was recently approved for clinical use in colorectal cancer (.). However, ang

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Peptide-Based Strategy for siRNA Delivery into Mammalian Cells, cells and in vivo (.). So far, although siRNA transfection can be achieved with classical laboratory-cultured cell lines using lipid-based formulations, siRNA delivery remains a major challenge for many cell lines and there is still no reasonably efficient method for in vivo application (.). The mo

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