Titlebook: RNA Scaffolds; Methods and Protocol Luc Ponchon Book 2021Latest edition Springer Science+Business Media, LLC, part of Springer Nature 2021

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Production of Circular Recombinant RNA in , Using Viroid Scaffolds,ccumulation most probably results from viroid scaffold stability, resistance to exonucleases due to circularity, and accumulation as a ribonucleoprotein complex with tRNA ligase. Purification of the recombinant RNA from total . RNA is also facilitated by the circular structure of the product.

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Aptazyme-Based Riboswitches and Logic Gates in Mammalian Cells,zymes can be cloned into the 3′ UTR of a desired gene to obtain a logic gate response to multiple chemical signals. This screening strategy complements other methods to engineer robust mammalian riboswitches to control gene expression.