Titlebook: RNA Remodeling Proteins; Methods and Protocol Marc Boudvillain Book 2015 Springer Science+Business Media New York 2015 DEAD-box Helicase.EM

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Bioinformatics and Biochemical Methods to Study the Structural and Functional Elements of DEAD-Box f interest, which we modify by classical molecular biological techniques (mutations and deletions). We then use various biochemical techniques to characterize the purified proteins and their variants for their ATPase, RNA binding, and RNA unwinding activities to determine the functional roles of the

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A Fluorescence-Based Screening Assay for Identification of Hepatitis C Virus NS3 Helicase Inhibitorh a fluorophore-labeled strand hybridized to a quencher-labeled strand and monitors the increase in fluorescence intensity resulting from helicase-catalyzed unwinding of the dsRNA substrate. We further describe radioactive assays to directly visualize RNA strands unwound by helicase and to evaluate

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https://doi.org/10.1007/978-1-4939-2214-7DEAD-box Helicase; EMOTE; Fluorescence Resonance Energy Transfer (FRET); RHAU; RNA Helicase; RNA targets;

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Determination of RNA Chaperone Activity Using an , Mutant,splays a cold-sensitive growth phenotype. Plant cold shock domain (CSD) proteins have been shown to complement the cold-sensitive phenotype of the . mutant and to share a function with . CSPs as RNA chaperones. This methodology, which is detailed here, can be utilized to reveal or probe the RNA chaperone activity of heterologous proteins.

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Probing RNA Translocases with DNA,manganate (KMnO.) footprinting is a method used to chemically probe the conformation of DNA as well as the binding of proteins. Combining footprinting methods with rapid mixing methods that utilize a chemical quench-flow instrument can enable tracking of the translocase with nucleotide resolution.

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RNA Remodeling Proteins978-1-4939-2214-7Series ISSN 1064-3745 Series E-ISSN 1940-6029