Titlebook: RNA Abundance Analysis; Methods and Protocol Hailing Jin,Isgouhi Kaloshian Book 2021Latest edition Springer Science+Business Media, LLC, pa

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Purification and Analysis of Chloroplast RNAs in ,,phere. Additionally, chloroplasts regulate energy production, metabolite synthesis, and stress responses in plants and algae. Chloroplasts possess a notably complex RNA metabolism that includes RNA processing, editing, splicing, and regulation by various RNA-binding proteins. Highly purified chlorop

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In Situ Detection of Mature miRNAs in Plants Using LNA-Modified DNA Probes, patterns. Therefore, in situ detection of mature miRNAs is important for understanding the functions for both miRNAs and their targets. The construction of promoter-reporter fusions and examination of their in planta expression has been widely used and the results obtained thus far are rather infor

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Northern Blotting Technique for Detection and Expression Analysis of mRNAs and Small RNAs,ence of a transcript but also indicates size and relative comparison of transcript abundance on a single membrane. In recent years it has been aptly adapted to validate and study the size and expression of small noncoding RNAs. Here, we describe protocols employed in our laboratory for conventional

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Small RNA Isolation and Library Construction for Expression Profiling of Small RNAs from , and , and are implicated in significant diseases and metabolic disorders. Elucidation of biogenesis mechanisms and functional characterization of sRNAs is often achieved using tools such as separation of small-sized RNA and deep sequencing. Although RNA interference pathways, such as quelling and meiotic si

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Book 2021Latest editionntional methods to state-of-the-art high throughput approaches. It includes detailed techniques to examine mRNAs, small non-coding RNAs, protein-associated small RNAs, organelle RNAs, endosymbiont RNAs, and alternatively spliced RNA variants from various organisms. RNA editing and the computational