Titlebook: Quantitation of mRNA by Polymerase Chain Reaction; Nonradioactive PCR M Thomas K?hler,Dirk La?ner,Barbara Pustowoit Book 1995 Springer-Verl

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Synthesis of cDNAFor detection or quantitation of mRNA by polymerase chain reaction, it is necessary to transcribe mRNA into copy-DNA (cDNA) by a reverse transcriptase (RT) reaction. The quality of a cDNA depends on the integrity of the messenger RNA and the fidelity of the transcription.

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Quantitation of Absolute Numbers of mRNA Copies in a cDNA Sample by Competitive PCRIn this chapter a competitive PCR assay using an internal DNA standard is described. Competitive PCR is a quantitative adaptation of the PCR method in which a known number of copies of an exogenous synthesized and added DNA (or RNA) is amplified together with the sample in the same PCR tube (see chapter 1.1).

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