| 書目名稱 | Pyrosequencing | | 副標(biāo)題 | Methods and Protocol | | 編輯 | Ulrich Lehmann,J?rg Tost | | 視頻video | http://file.papertrans.cn/765/764201/764201.mp4 | | 概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts | | 叢書名稱 | Methods in Molecular Biology | | 圖書封面 |  | | 描述 | .The primary purpose of this volume is to demonstrate the range of applications of the Pyrosequencing technology in research and diagnostics and to provide detailed protocols. Beginning with an up-to-date overview of the biochemistry, the volume continues with quantitative analysis of genetic variation, ratio of expressed alleles at the RNA level, analysis of DNA methylation, global DNA methylation assays, specialized applications for DNA methylation analysis including loss of imprinting, single blastocyst analysis, allele-specific DNA methylation patterns, DNA methylation patterns associated with specific histone modifications. The volume further details tools and protocols for the detection of viruses and bacteria, and genetic and epigenetic analyses for forensics using Pyrosequencing. As a volume in the highly successful .Methods in Molecular Biology. series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and tips on troubleshooting and avoiding known pitfalls..Comprehensive and adaptable, .Pyrosequencing: Methods and Protocols, Second Edition. will greatly aid doctorial stude | | 出版日期 | Book 2015Latest edition | | 關(guān)鍵詞 | DNA methylation; HLA alleles; Pyrograms; allele-specific DNA methylation patterns; alleles; chromosomal a | | 版次 | 2 | | doi | https://doi.org/10.1007/978-1-4939-2715-9 | | isbn_softcover | 978-1-4939-4909-0 | | isbn_ebook | 978-1-4939-2715-9Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Springer Science+Business Media New York 2015 |
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