| 書目名稱 | Protein NMR Techniques | | 編輯 | A. Kristina Downing | | 視頻video | http://file.papertrans.cn/762/761722/761722.mp4 | | 概述 | Includes supplementary material: | | 叢書名稱 | Methods in Molecular Biology | | 圖書封面 |  | | 描述 | When I was asked to edit the second edition of Protein NMR Techniques, my first thought was that the time was ripe for a new edition. The past several years have seen a surge in the development of novel methods that are truly revolutionizing our ability to characterize biological macromolecules in terms of speed, accuracy, and size limitations. I was particularly excited at the prospect of making these techniques accessible to all NMR labs and for the opportunity to ask the experts to divulge their hints and tips and to write, practically, about the methods. I commissioned 19 chapters with wide scope for Protein NMR Techniques, and the volume has been organized with numerous themes in mind. Chapters 1 and 2 deal with recombinant protein expression using two organisms, E. coli and P. pastoris, that can produce high yields of isotopically labeled protein at a reasonable cost. Staying with the idea of isotopic labeling, Chapter 3 describes methods for perdeuteration and site-specific protonation and is the first of several chapters in the book that is relevant to studies of higher molecular weight systems. A different, but equally powerful, method that uses molecular biology to “edit” | | 出版日期 | Book 2004Latest edition | | 版次 | 2 | | doi | https://doi.org/10.1385/1592598099 | | isbn_softcover | 978-1-61737-449-4 | | isbn_ebook | 978-1-59259-809-0Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Humana Press 2004 |
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