| 書目名稱 | Plant Cell Electroporation And Electrofusion Protocols | | 編輯 | Jac A. Nickoloff | | 視頻video | http://file.papertrans.cn/749/748486/748486.mp4 | | 叢書名稱 | Methods in Molecular Biology | | 圖書封面 |  | | 描述 | Gene transfer is an essential technology for improving our under- standing of gene structure and function. Although there are many meth- ods by which DNA may be introduced into cells—including heat and chemical treatments, and microinjection—electroporation has been found to be the most versatile gene transfer technique. Electroporation is effective with a wide variety of cell types, including those that are difficult to transform by other means. For many cell types, electroporation is either the most efficient or the only means known to effect gene transfer. The early and broad success of electric field-medi- ated DNA transfer soon prompted researchers to investigate electroporation for transferring other types of molecules into cells, in- cluding RNA, enzymes, antibodies, and analytic dyes. The first section of Plant Cell Electroporation and Electrofusion Protocols includes two chapters that serve as a guide to theoretical and practical aspects of electroporation, and will be of particular interest to those developing protocols for as yet untested species or cell types, and a third chapter that describes commercially available electroporation instruments. The remaining chapters d | | 出版日期 | Book 1995 | | 版次 | 1 | | doi | https://doi.org/10.1385/0896033287 | | isbn_softcover | 978-1-4899-4080-3 | | isbn_ebook | 978-1-59259-542-6Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Springer Science+Business Media New York 1995 |
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