Titlebook: Optogenetics; Methods and Protocol Arash Kianianmomeni Book 2016 Springer Science+Business Media New York 2016 algal sensory photoreceptors

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Methods in Molecular Biologyhttp://image.papertrans.cn/o/image/703455.jpg

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978-1-4939-8062-8Springer Science+Business Media New York 2016

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Arash KianianmomeniIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia

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Reversible Photoregulation of Gene Expression and Translation,ntrolling gene expression, although typically in an irreversible manner. We here describe a new approach for reversibly controlling translation using a photoresponsive 8-styryl cap (8ST-cap) that can be reversibly isomerized by illumination with light of a specific wavelength.

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Photo Control of Protein Function Using Photoactive Yellow Protein,designing new tools to manipulate biological processes, but with respect to other protein scaffolds it presents some technical challenges. Here, we present practical information on how to overcome these, including how to synthesize the PYP chromophore, how to express and purify PYP, and how to screen for desired activity.