Titlebook: Opioid Receptors; Methods and Protocol Santi M. Spampinato Book 2021Latest edition Springer Science+Business Media, LLC, part of Springer N

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Immunohistochemical Analysis of Opioid Receptors in Peripheral Tissues bodies, along the axons and their peripheral endings in the hind paw skin, as well as in the spinal cord, under na?ve and sciatic nerve damage conditions in mice. Importantly, we consider the ongoing debate on the specificity of antibodies.

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Patch?Clamp Analysis of Opioid-Induced Kir3 Currents in Mouse Peripheral Sensory Neurons Following Nonic constriction injury (CCI) of the sciatic nerve, which models neuropathic pain. We describe the induction of the CCI, isolation and culture of DRG neurons, performance of the patch?clamp recordings, and identification of opioid-responding neurons.

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Overview of Genetic Analysis of Human Opioid Receptorss. The μ-opioid receptor (MOR), encoded by OPRM1, contributes to regulate the analgesic response to pain and also controls the rewarding effects of many drugs of abuse, including opioids, nicotine, and alcohol. Genetic polymorphisms of opioid receptors are candidates for the variability of clinical

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