Titlebook: Oligonucleotide-Based Therapies; Methods and Protocol Olof Gissberg,Rula Zain,Karin E. Lundin Book 2019 Springer Science+Business Media, LL

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Measuring the Action of Oligonucleotide Therapeutics in the Lung at the Cell Type-Specific Level by s from cell culture to in vivo models and the clinic. For example, classical preclinical approaches routinely report 20–60% target knockdown effects in the lung, where 1 or 2 log effects are observed in isolated cell cultures in vitro. Preparation of monocellular suspensions of tissues by mechanoenz

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Delivery of Antisense Oligonucleotides Mediated by a Hydrogel System: In Vitro and In Vivo Applicatie or RNA interference based drugs, as they can potentially reduce systemic toxicity and enhance in vivo efficacy by increasing in situ concentrations. Here we describe the preparation of antisense oligonucleotide-loaded fibrin hydrogels exploring their applications in the context of the nervous syst

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Evaluation of Cell-Penetrating Peptide Delivery of Antisense Oligonucleotides for Therapeutic Efficacy is its bioavailability for in vivo and in vitro studies. To overcome this challenge we use cell-penetrating peptides (CPPs) for systemic delivery of ASOs. One of the most advanced clinical uses of ASOs is for the treatment of spinal muscular atrophy (SMA). In this chapter, we describe the techniq

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In Vitro Assessment of the Hepatotoxicity Potential of Therapeutic Oligonucleotidesmostly investigated directly in rodent studies. A predictive preclinical in vitro model that is capturing liver liabilities of antisense oligonucleotides can be of great help to be used as a first filter in the screening process of therapeutic oligonucleotides. We describe here an in vitro cytotoxic

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Karin E. Lundin,Olof Gissberg,C. I. Edvard Smith,Rula Zain-30% der Unternehmen elektronische Marktpl?tze nutzen [BaHP03; EUCO03, 28; EUCO05a, 30; GNEK06, 363 ff., 397; PROZ05, 3] und darüber hinaus weitere Unternehmen in den kommenden Jahren deren Einsatz planen [OBKM02, 33; PROZ05, 3]. Insgesamt gehen Unternehmen von einer intensiveren Nutzung elektronisc