Titlebook: Oat; Methods and Protocol Sebastian Gasparis Book 2017 Springer Science+Business Media LLC 2017 Avenae.Hexaploid oat.amino acids.genetic en

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Protocol for Producing Synthetic Polyploid Oatsbetween . species. Furthermore, artificial hybridization procedures between different ploidy species are explained. Amphiploids can be produced by rescuing aborted embryos and treating the F. hybrids with colchicine to overcome sterility between interspecific plants. Furthermore, I present the cytol

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Oat Anther Culture and Use of DH-Lines for Genetic Mappinge material for genetic research. Oats is reported to be recalcitrant in anther culture with low response and genotype dependency. However, the best recoveries reported have reached up to 30 green regenerants per 100 isolated anthers, which clearly addresses the potential of this technique. In this c

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-Mediated Transformation of Leaf Base Segmentson patterns of transgenic loci, decent transformation efficiency, and technical simplicity are the main advantages offered by this method. Here we present a detailed protocol for the production of transgenic oat plants by .-mediated transformation of leaf base segments. The use of leaf explants as t

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Microarray-Based Immunoassay for Parallel Quantification of Multiple Mycotoxins in Oatllel analysis of several mycotoxins. Here, we describe an indirect competitive immunoassay on regenerable, reusable glass microchips for the parallel determination of aflatoxins, ochratoxin A, deoxynivalenol, and fumonisin B1 in oat extracts, using a fully automated flow-through device with chemilum

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M13-Tailed Simple Sequence Repeat (SSR) Markers in Studies of Genetic Diversity and Population Struc is due to their uniform distribution in the genome, the high polymorphism, reproducibility, and codominant character. Additional advantages are the possibility of automatic analysis and simple interpretation of the results. The M13 tagged PCR reaction significantly reduces the costs of analysis by

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Genotyping-by-Sequencing and Its Application to Oat Genomic Research mapping, and conducting genomic selection. It is a combined one-step process of SNP marker discovery and genotyping through genome reduction with restriction enzymes and SNP calling with or without a sequenced genome. This approach has the advantage of being rapid, high throughput, cost effective,