Titlebook: Nuclear Reprogramming; Methods and Protocol Steve Pells Book 2006 Humana Press 2006 Activation.DNA.Ethylen.Monoclonal Antibodies.PCR.Telome

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Nuclear Reprogramming by Cell Fusion,m switching is mediated by .-acting factors. Other possible uses of this cell fusion protocol in stem cell biology and transplantation of genetically engineered cells for tissue regeneration are briefly discussed.

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Epigenetic Reprogramming of Somatic Genomes by Electrofusion With Embryonic Stem Cells,clei. Interestingly, nuclear reprogramming for conferring pluripotency on somatic nuclei also occurs via cell fusion between pluripotential stem cells and somatic cells. Furthermore, it has been shown that spontaneous cell fusion contributes to generating the intrinsic plasticity of tissue stem cell

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Quantitative Analysis of Telomerase Activity and Telomere Length in Domestic Animal Clones,hromosome capping. Telomere-mediated signaling of cellular senescence has been established for many somatic cell types in vitro, except for germ cells, cancer lines, and regenerative tissues in which telomere length is maintained primarily by the ribonucleoprotein telomerase, a reverse transcriptase

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Staining Embryonic Stem Cells Using Monoclonal Antibodies to Stage-Specific Embryonic Antigens,res; (2) examine the spatial distribution of stem cells and differentiated derivatives in monolayer cultures and embryoid bodies; and (3) isolate populations of differentiated and undifferentiated cells by immunomagnetic or fluorescence-activated cell sorting for cellular and molecular analyses.

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Methylation-Sensitive Polymerase Chain Reaction,um bromide-stained agarose gel is proportional to the amount of methylated DNA in each embryo. By comparing quadruplicate values obtained for each embryo against a standard curve, we are able to ensure the validity of our results for each individual embryo. Compared with bisulphite sequencing method