Titlebook: Nuclear Bodies and Noncoding RNAs; Methods and Protocol Shinichi Nakagawa,Tetsuro Hirose Book 2015 Springer Science+Business Media New York

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RNA Antisense Purification (RAP) for Mapping RNA Interactions with Chromatinntal procedures for RNA antisense purification (RAP), a method for selective purification of endogenous RNA complexes from cell extracts that enables mapping of RNA interactions with chromatin. In RAP, the user cross-links cells to fix endogenous RNA complexes and purifies these complexes through hy

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Extracting, Enriching, and Identifying Nuclear Body Sub-Complexes Using Label-Based Quantitative Masclear body preparations. Moreover, many nuclear proteins dynamically associate with multiple bodies and subnuclear compartments, confounding analysis. We have found that a more practical approach is to carry out affinity purification of nuclear body sub-complexes via the use of tagged nuclear-body-s

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