Titlebook: Nonradioactive Labeling and Detection of Biomolecules; Christoph Kessler Book 19921st edition Springer-Verlag Berlin Heidelberg 1992 Aspar

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James G. Lazar,Floyd E. Taubnted the opportunity to study innovation processes from the Centre for Cooperative Studies at the Norwegian School of Management BI, the main agricultural food cooperatives in Norway — who were sponsors of the centre — became the most relevant empirical fields of study. — that is, if I could find relevant and interesting cases there.

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nted the opportunity to study innovation processes from the Centre for Cooperative Studies at the Norwegian School of Management BI, the main agricultural food cooperatives in Norway — who were sponsors of the centre — became the most relevant empirical fields of study. — that is, if I could find relevant and interesting cases there.

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General Aspects of Nonradioactive Labeling and Detectiontive bioanalytical indicator systems have been made. In addition to special techniques such as nucleic acid detection via ethidium bromide intercalation (Bauer and Vinograd, 1968; Nathans and Smith, 1975; Ausubel et al., 1987), protein visualization by Coomassie staining (Bennett and Scott, 1971; Ze

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Overview of Nonradioactive Labeling Systemsrtant labeling and detection systems, including cross-references to the descriptions in the other chapters and sections in this book. Among the indirect approaches the most sensitive systems are: (a) the biotin:(sttept-) avidin (bio) and (b) the digoxigenin:anti-digoxigenin (DIG) system which realiz

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Colloidal Gold as a Marker in Molecular Biology: The Use of Ultra-Small Gold Probesare gold sols in a wide range of sizes, with a narrow size distribution enabling double or even triple labeling in electron microscopy (EM). A large variety of macromolecules other than antibodies (e.g., streptavidin, protein A, lectins, enzymes, and ligands such as insulin) were coupled to colloida

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Direct Peroxidase Labeling of Hybridization Probes and Chemiluminescence DetectionKurz, 1984). Single-stranded nucleic acid (RNA or DNA) is labeled with a positively charged, modified, horseradish peroxidase (HRP) complex in a rapid, reliable, and simple reaction process to produce a stable probe. In conjunction with enhanced chemiluminescence (ECL), a light-based detection syste

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A Highly Sensitive Method for Detecting Peroxidase in In Situ Hybridization or Immunohistochemical A with counterstain, fading of chromogen with time or exposure to solvents, and inability to detect signals present at low levels. A highly sensitive procedure for peroxidase detection in tissue sections and cellular samples is described (Taub and Higgs 1991). The peroxidase label may be attached dir

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The SNAP Systemr cloned DNA probes. Highly specific oligonucleotides can distinguish between sequences with as few as one nucleotide difference (Wallace et al., 1979). Automated chemistry has enabled the routine production of high purity oligonucleotides using phosphoamidite chemistry. The development of SNAP (syn