Titlebook: Neuronal Morphogenesis; Methods and Protocol Kazuhito Toyooka Book 2024 The Editor(s) (if applicable) and The Author(s), under exclusive li

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congenial

In Ovo Electroporation of Embryonic Chicken Spinal Cord in Combination with Ex Vivo Slice Culture f their native tissue environment. Here, we describe an accessible protocol for fluorescent live imaging of differentiating neurons within ex vivo embryonic chicken spinal cord slice cultures, which facilitates long-term observation of individual cells within developing tissue.

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In Vitro and In Vivo Analysis of Neuronal Polarity,tical step during development that allows the specification of the different neuronal processes as a single axon and multiple dendrites both structurally and functionally, allowing the unidirectional flow of information. Along with extrinsic and intrinsic signaling, a whole network of molecular comp

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DNeuroMAT: A Deep-Learning-Based Neuron Morphology Analysis Toolbox,nctions. Currently, neuron reconstructions are mainly obtained by manual or semiautomatic methods. However, these ways are labor-intensive, especially when handling the huge volume of whole brain microscopy imaging data. Here, we present a .eep-learning-based .n .orphology .nalysis .oolbox (DNeuroMA

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Ex Vivo Live Imaging in Brain Slices for Analysis of Neurite Formation in Combination with In Uterolices provides a more physiological environment than cultured cells. To accomplish this, a certain method of labeling is necessary to visualize and identify neurite morphology. To understand the dynamics of neurite structure at early stages of neurite formation, we describe in this chapter ex vivo l

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Imaging of Structural Plasticity of Dendritic Spines with Two-Photon Microscopy,ed structural plasticity. Therefore, understanding the mechanism of structural plasticity is critical for understanding the mechanism of synaptic plasticity. In this chapter, we describe the procedures and equipment required to image structural plasticity of a single dendritic spine, which hosts exc

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growth-factor