Titlebook: Natural Killer Cells; Methods and Protocol Srinivas S. Somanchi Book 2016 Springer Science+Business Media New York 2016 cytotoxicity.genoty

感染

instill

High- and Super-Resolution Microscopy Imaging of the NK Cell Immunological Synapse,nts and the role they play in the regulation and execution of NK cell directed secretion. Here we describe a protocol for the recapitulation of the NK cell lytic synapse on glass, the acquisition of microscopy images, and suggested approaches for the processing and analysis of microscopy data.

labile

Tracking Effector and Memory NK Cells During MCMV Infection,s with MCMV. Here we describe an adoptive transfer model in which the expansion, contraction, and memory cell persistence of transferred Ly49H. NK cells are tracked in congenic C57BL/6 hosts following MCMV infection.

宏偉

Assessment of NK Cell Metabolism, assessment of real-time glycolysis and oxidative phosphorylation by an extracellular flux assay from Seahorse Biosciences, and some basic protocols for stimulation of NK cells via cytokines and receptors.

偉大

ostrish

Book 2016new developments in the natural killer (NK) cell field, such as understanding the influence of NK cells metabolism on its function, identifying complexity of NK cell subsets through mass cytometry, and determining the emergence of memory NK cells in murine model of MCMV infection. Methods that study

infarct

Ex Vivo Expansion of Human NK Cells Using K562 Engineered to Express Membrane Bound IL21,K562 mb.IL21). We had previously demonstrated that this platform enables the highest fold expansion of NK cells reported in the literature to date (>47,000-folds in 21 days), and produces highly activated and pure NK cells without signs of senescence, as determined by telomere shortening.

矛盾

Abrade

特別容易碎

Analysis of Intracellular Ca2+ Mobilization in Human NK Cell Subsets by Flow Cytometry,pecific NK cell subsets is possible. Here, a flow cytometric method for dynamic measurements of intracellular Ca. concentrations in human NK cells subsets is detailed and discussed. This assay can be further adapted for specific scientific and diagnostic questions, with implications for various immunopathological conditions.