Titlebook: Nanopore Sequencing; Methods and Protocol Kazuharu Arakawa Book 2023 The Editor(s) (if applicable) and The Author(s), under exclusive licen

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Ultralow-Input Genome Library Preparation for Nanopore Sequencing with Droplet MDAencing is a promising technology for genome assembly due to its limitless long reads, recommended input of 1?μg for the Ligation Sequencing Kit often precludes the use of this technology. Here, I detail an unbiased droplet-based multiple displacement amplification of picogram order of DNA to realize

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Analysis of Tandem Repeat Expansions Using Long DNA Readsdisease-causing repeats in the human genome. Long read sequencers enable us to directly analyze repeat length and sequence content by covering whole repeats; they are therefore considered suitable for the analysis of long tandem repeats. Here, we describe an expanded repeat analysis using target seq

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Long-Read Whole-Genome Sequencing Using a Nanopore Sequencer and Detection of Structural Variants in of diseases, including cancers. In this section, we introduce experimental and computational procedures for conducting long-read whole-genome sequencing (WGS) of cancer genomes from fresh frozen tissues/cells. We also demonstrate the analysis of SVs in cancer genomes using long-read WGS data from l

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Nanopore Sequencing Data Analysis of 16S rRNA Genes Using the GenomeSync-GSTK Systemintroduces the GenomeSync-GSTK system for bacterial species identification in a given sample using nanopore sequencing data of 16S rRNA genes as an example. GenomeSync is a collection of genome sequences designed to provide easy access to genomic data of the species as demanded. GSTK (genome search

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