Titlebook: Microglia; Methods and Protocol Olga Garaschuk,Alexei Verkhratsky Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2

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Ultrastructural Analyses of Microglial Interactions with Synapsesnize based on their ultrastructural features. The technique has been essential in defining, at high spatial resolution, microglial interactions with neurons and synapses, thus providing, among other discoveries, important insights into their roles in synaptic pruning and stripping. In this protocol,

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Replenishment of Organotypic Hippocampal Slice Cultures with Neonatal or Adult Microgliare preparation from newborn mice, standard culturing of neonatal microglia, and the acute isolation of microglia from adult mouse brain. Furthermore, we outline the technique for the replenishment of microglia-depleted slice cultures with different microglia populations and subsequent morphological

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Long-Term In Vivo Imaging of Individual Microglial Cells the parenchyma, and swiftly responding to a variety of external stimuli. Capturing the dynamics of their morphology, reaction to trauma, pathogens, or endogenous stimuli, and studying changes in their network in their physiological environment requires the use of two-photon microscopy, as well as a

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