Titlebook: Microbial Production; From Genome Design t Hideharu Anazawa,Sakayu Shimizu Book 2014 Springer Japan 2014 Minimum genome factory.Molecular b

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Development and Application of Novel Genome Engineering Technologies in ,aim of PCS is to cut a chromosome at any chosen site into two smaller pieces and to make these “newly generated chromosomes” behave as functional chromosomes. PCS splits a chromosome very efficiently (more than 70 %) and allows repeated splitting because the built-in Cre/.P site-specific recombinati

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Genome Design of Actinomycetes for Secondary Metabolismogous expression of genes encoding secondary metabolite biosynthesis. Several kinds of the entire biosynthetic gene clusters for secondary metabolites were successively cloned and introduced into a versatile model host, .. Most . transformants carrying the entire gene cluster produced metabolite(s)

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