Titlebook: Metastasis Research Protocols; Miriam Dwek,Susan A. Brooks,Udo Schumacher Book 2012Latest edition Springer Science+Business Media, LLC 201

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Immunocytochemical Detection and Characterisation of Individual Micrometastatic Tumour Cells,y employed across different laboratories, however, renders comparison of results difficult. This chapter describes a robust reliable system for the immunochemical detection of individual micrometastatic tumour cells from the BM aspirates of patients with solid epithelial tumours, based on the bindin

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The Polymerase Chain Reaction,. The sensitivity and specificity inherent in the technique allow minute quantities of genetic material to be detected while the unique properties of thermostable DNA polymerase ensure that abundant copies are reliably reproduced to levels that can be visualized and/or used for further applications.

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Assessment of Gelatinases (MMP-2 and MMP-9) by Gelatin Zymography,articularly useful for the assessment of two key members of the matrix metalloproteinase family, MMP-2 (gelatinase A) and MMP-9 (gelatinase B), due to their potent gelatin-degrading activity. This polyacrylamide gel electrophoresis-based method can provide a reliable assessment of the type of gelati

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Determination of Cell-Specific Receptor Binding Using a Combination of Immunohistochemistry and In l mechanisms. Such information may potentially be translated into targeted therapies, especially in the field of cancer treatment. In this chapter, a receptor localization technique is described which utilises frozen sections of human tissue and combines immunohistochemistry (IHC) and micro-autoradi

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