Titlebook: Metabolic Pathway Engineering; Michael E. Himmel,Yannick J. Bomble Book 2020 Springer Science+Business Media, LLC, part of Springer Nature

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Measuring Biomass-Derived Products in Biological Conversion and Metabolic Process,titative and qualitative information of products in these conversion processes are essential. Here we introduce analytical techniques including high-performance liquid chromatography (HPLC), gas chromatography (GC), gas chromatography-mass spectrometry (GC-MS), and nuclear magnetic resonance (NMR) f

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Measuring Metabolic Enzyme Performance,tive enzymes and heterologously expressed enzymes that play key roles in pathway performance (Zeldes et al., Front Microbiol 6:1209, 2015; Keller et al., Metab Eng 27:101–106, 2015). This step cannot be overlooked as gene expression is not always a good indicator of the production of fully active en

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Gene Editing Technologies for Biofuel Production in Thermophilic Microbes,ble enzymatic processes which may be required for biomass processing and other industrial applications. The engineering of microbes for industrial scale processes requires a suite of genetic engineering tools to optimize existing biological systems as well as to design and incorporate new metabolic

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Connecting Microbial Genotype with Phenotype in the Omics Era,as adaptive laboratory evolution (ALE) and mutagenesis are still efficient approaches to improve microorganisms for exceptional features such as a broad spectrum of substrate utilization, robustness of cell growth, as well as high titer, yield, and productivity of the target products. In this chapte

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