Titlebook: Marijuana and Cannabinoid Research; Methods and Protocol Emmanuel S. Onaivi Book 2006 Humana Press 2006 cannabinoid.clinical research.depre

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Localization of Cannabinoid Receptors Using Immunoperoxidase Methods,s also present at lower levels in other tissues. The second receptor, CB., has been found natively in cells of the immune system. Both receptors have an extracellular amine-terminal domain, seven transmembrane domains, an intracellular carboxy-terminal domain, and are coupled through G proteins to a

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Localization of Cannabinoid CB1 Receptor mRNA Using Ribonucleotide Probes,ying the co-localization of cannabinoid CB. receptor mRNA with other distinct species of mRNAs. This method permits simultaneous detection of two different species of mRNA within the same tissue section. Double-label ISH may be accomplished by hybridizing tissue sections with a combination of radiol

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Methods for the Synthesis of Cannabinergic Ligands,m rigorously pursued structure-activity relationship studies. This chapter focuses on the synthetic aspects of key cannabinoid receptor probes representing the different classes of cannabinergic ligands that encompasses classical cannabinoids (CCs) including some covalent binding derivatives, noncla